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Light microscopy

M.A.S… examines your samples under: in conjunction with digital image analysis.

Reflected light

The specimen is illuminated from above or from the side. Thus, the surface or superficial regions of the sample can be seen.

Typical illumination modes in incident light microscopy are:
  • Bright Field
  • Dark field
  • Polarization
  • Differential interference contrast
  • Fluorescence
  • Phase-contrast

Cross-section through a seal

Transmission light microscopy

With this method, light shines through the specimen. The light should shine through the sample, thus, it is either transparent or is thinly dissected accordingly.

Typical illumination modes in transmission light microscopy are:
  • Bright Field
  • Dark field
  • Polarization

Ion exchanger micro spheres

Polarized light microscopy

Polarized light is used for imaging the sample (e.g. with minerals or crystals).

To this end, two polarizing filters, one of which is turnable, are placed in the beam path of the microscope.

Layer structure of a packaging foil

Fluorescence microscopy

The specimen is either autofluorescent or is tagged accordingly with fluorescent materials (fluorochromes) for the examination. Using light of a specific wavelength, it is then stimulated to glow.

The wavelength necessary for the excitation of the fluorescent dye is generated and routed onto the sample by means of optical filters. Subsequently, the fluorescing areas are imaged.

Cell with different fluorescence excitation

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